rabbit anti ep300  (Cell Signaling Technology Inc)

Journal: European Journal of Medical Research

Article Title: Long non-coding RNA NORAD serves as a promoter of oncogenesis and inhibits ferroptosis via miR-144-3p-mTOR-ferritinophagy axis in cancer

doi: 10.1186/s40001-025-02998-2

Figure Lengend Snippet: Histone acetylation is positively related with NORAD expression. A Enrichment of H3K27ac at the up-stream of NORAD in individual types of cancer cells. B – I Histone acetyltransferase EP300 is positively related with NORAD expression in LUAD ( B ), LUSC ( C ), LIHC ( D ), CESC ( E ), CHOL ( F ), COAD ( G ), DLBC ( H ) and ESCA ( I ). ENCODE -s applied to obtain the data of H3K27ac at upstream of NORAD. Correlation between EP300 and NORAD was obtained from TCGA database

Article Snippet: The sheared chromatin was immunoprecipitated with anti-EP300 antibody (CA: 86377, Cell Signaling Technology, USA) at 4 °C overnight.

Techniques: Expressing

Journal: European Journal of Medical Research

Article Title: Long non-coding RNA NORAD serves as a promoter of oncogenesis and inhibits ferroptosis via miR-144-3p-mTOR-ferritinophagy axis in cancer

doi: 10.1186/s40001-025-02998-2

Figure Lengend Snippet: Influence of ferroptosis inducers (chemotherapeutic agents and radiation) to NORAD expression. A , B RT-PCR detected expression of NORAD under different agents application in A549 cells ( A ) and PC9 ( B ) cells (cisplatin, 10 μM, 24 h, gemcitabine, 25 μg/ml, 24 h, pemetrexed, 10 μM, 24 h, docetaxel, 40 nM, 24 h, an equal volume of DMSO was used as the control). C RT-PCR detected expression of NORAD under sorafenib application (5 μM, 24 h, an equal volume of DMSO was used as the control) in SMMC-7721 cells. D – F RT-PCR detected expression of NORAD under radiation (2 Gy, 4 Gy and 8 Gy) in A549 ( D ), PC9 ( E ) and SMMC-7721 ( F ) cells. G Western-Blot detected expression of EP300 under treatment of cisplatin (10 μM, 24 h, an equal volume of DMSO was used as the control). H , I ChIP-PCR detected alteration of combining capacity of EP300 to NORAD under cisplatin treatment (10 μM, 24 h, an equal volume of DMSO was used as the control). *** P < 0.001, NS no significance. Data represents at independent experiments

Article Snippet: The sheared chromatin was immunoprecipitated with anti-EP300 antibody (CA: 86377, Cell Signaling Technology, USA) at 4 °C overnight.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Western Blot